Profile

Education

Ph.D. (2004-2012)

The Institute of Basic Medical Science, National Cheng Kung University, Tainan, Taiwan

M.S. (2002-2004)

Department of Microbiology and Immunology, National Cheng Kung University, Tainan, Taiwan

B.S. (1998-2002)

Department of Medical Technology, Kaohsiung Medical University, Kaohsiung, Taiwan

Professional experience

2021-present       Assistant Professor, Department of Microbiology and Immunology, National Cheng Kung University, Tainan, Taiwan

2017-2021           Assistant Professor, School of Medicine for International Students, I-Shou University, Kaohsiung, Taiwan

2012-2017           Postdoctoral Fellow, Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan

Specialties

Immunology

Virology

Cell biology

Research interest

Dengue virus (DENV) infection is one of the leading causes of arthropod-borne viral disease. Up till now, there are no licensed antiviral drugs or therapeutics for DENV treatment. The viral protein, non-structural protein 1 (NS1) plays a key factor during DENV infection, with intracellular roles in viral RNA replication and virion production as well as extracellular roles in plasma leakage and immune evasion. Therefore, my research interests focus on the pathogenesis and potential clinical application of NS1.

The main direction of my laboratory is:

• The roles of dengue virus NS1 on regulating cellular homeostasis.
• The secretory pathways of NS1.
• Development of dengue virus NS1-based subunit vaccine and therapeutic antibody for effective and extensive application.

Publication

Refereed papers

1. Tien SM, Chang PC, Lai YC, Chuang YC, Tseng CK, Kao YS, Huang HJ, Hsiao YP, Liu YL, Lin HH, Chu CC, Cheng MH, Ho TS, Chang CP, Ko SF, Shen CP, Anderson R, Lin YS*, Wan SW*, Yeh TM*. Therapeutic efficacy of humanized monoclonal antibodies targeting dengue virus nonstructural protein 1 in the mouse model. PLoS Pathog 2022; 18: e1010469. (*equal contribution as the corresponding author)
2. Huang HJ, Yang M, Chen HW, Wang S, Chang CP, Ho TS, Kao YS, Tien SM, Lin HH, Chang PC, Lai YC, Hsiao YP, Liu YL, Chao CH, Anderson R, Yeh TM, Lin YS*, Wan SW*. A novel chimeric dengue vaccine candidate composed of consensus envelope protein domain III fused to C-terminal-modified NS1 protein. Vaccine 2022; 40: 2299-2310. (*equal contribution as the corresponding author)
3. Wan SW, Lee YR, Ho TS, Chang CP. Regulation of innate immune signaling pathways by autophagy in dengue virus infection. IUBMB Life 2022; 74:170-179. (SCI)
4. Chang CJ, Lin CF, Lee CH, Chuang HC, Shih FC, Wan SW, Tai C, Chen CL. Overcoming interferon (IFN)-γ resistance ameliorates transforming growth factor (TGF)-β-mediated lung fibroblast-to-myofibroblast transition and bleomycin-induced pulmonary fibrosis. Biochem Pharmacol 2021; 183:114356. (SCI)
5. Lu ZY, Cheng MH, Yu CY, Lin YS, Yeh TM, Chen CL, Chen CC, Wan SW*, Chang CP*. Dengue nonstructural protein 1 maintains autophagy through retarding caspase-mediated cleavage of beclin-1. Int J Mol Sci 2020; 21:9702. (*equal contribution as the corresponding author) (SCI)
6. Kao YS, Yu CY, Huang HJ, Tien SM, Wang WY, Yang M, Anderson R, Yeh TM, Lin YS*, Wan SW*. Combination of modified NS1 and NS3 as a novel vaccine strategy against dengue virus infection. J Immunol 2019; 203:1909-1917. (*equal contribution as the corresponding author) (SCI)
7. Tseng PC, Kuo CF, Cheng MH, Wan SW, Lin CF, Chang CP, Lin YS, Wu JJ, Huang CC and Chen CL. HECT E3 ubiquitin ligase-regulated Txnip degradation facilitates TLR2-mediated inflammation during group A streptococcal infection. Front Immunol 2019; 10:2147. (SCI)
8. Wan SW, Wu-Hsieh BA, Lin YS, Chen WY, Huang Y, Anderson R. The monocyte-macrophage-mast cell axis in dengue pathogenesis. J Biomed Sci 2018; 25:77. (invited review article) (SCI)
9. Chiu YH, Chen MC, Wan SW. Sodium hyaluronate/chitosan composite microneedles as a single-dose intradermal immunization system. Biomacromolecules 2018; 19:2278-2285. (SCI)
10. Wan SW*, Chen PW*, Chen CY, Lai YC, Chu YT, Hung CY, Lee H, Wu H, Chuang YC, Lin J, Chang CP, Wang S, Liu CC, Ho TS, Lin CF, Lee CK, Anderson R, Yeh TM, Lin YS. Therapeutic effects of monoclonal antibody against dengue virus NS1 in a STAT1 knockout mouse model of dengue infection. J Immunol 2017; 199:2834-2844. (*equal contribution as the 1st author) (SCI)
11. Chu YT*, Wan SW*, Chang YC, Lee CK, Wu-Hsieh BA, Anderson R, Lin YS. Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation. Lab Invest 2017; 97: 602-617. (*equal contribution as the 1st author) (SCI)
12. Zainal N, Chang CP, Cheng YL, Anderson R, Wan SW, Ho TS, AbuBakar S, Lin YS. Resveratrol treatment reveals a novel role for HMGB1 in regulation of the type 1 interferon response in dengue virus infection. Sci Rep 2017; 7: 42998. (SCI)
13. Wan SW*, Yang YW*, Lin CF, Chang CP, Yeh TM, Anderson R, Lin YS. Anti-dengue virus nonstructural protein 1 antibodies contribute to platelet phagocytosis by macrophages. Thromb Haemost 2016; 115: 646-656. (*equal contribution as the 1st author) (SCI)
14. Chu YT, Wan SW, Anderson R, Lin YS. Mast cell-macrophage dynamics in modulation of dengue virus infection in skin. Immunology 2015; 146: 163-172. (SCI)
15. Cheng YL, Lin YS, Chen CL, Wan SW, Ou YD, Yu CY, Tsai TT, Tseng PC, Lin CF. Dengue virus infection causes the activation of distinct NF-κB pathways for inducible nitric oxide synthase and TNF-α expression in RAW264.7 cells. Mediat Inflamm 2015; 2015:274025. (SCI)
16. Cheng HJ, Luo YH, Wan SW, Lin CF, Wang ST, Hung NT, Liu CC, Ho TS, Liu HS, Yeh TM, Lin YS. Correlation between serum levels of anti-endothelial cell autoantigen and anti-dengue virus nonstructural protein 1 antibodies in dengue patients. Am J Trop Med Hyg 2015; 92: 989-995. (SCI)
17. Tsai TT, Chuang YJ, Lin YS, Chang CP, Wan SW, Lin SH, Chen CL, Lin CF. Antibody-dependent enhancement infection facilitates dengue virus-regulated signaling of IL-10 production in monocytes. PLoS Negl Trop Dis 2014; 8: e3320. (SCI)
18. Fang YT, Wan SW, Lu YT, Yao JH, Lin CF, Hsu LJ, Brown MG, Marshall JS, Anderson R, Lin YS. Autophagy facilitates antibody-enhanced dengue virus infection in human pre-basophil/mast cells. PLoS One 2014; 9: e110655. (SCI)
19. Wan SW, Lu YT, Huang CH, Lin CF, Anderson R, Liu HS, Yeh TM, Yen YT, Wu-Hsieh BA, Lin YS. Protection against dengue virus infection in mice by administration of antibodies against modified nonstructural protein 1. PLoS One 2014; 9: e92495. (SCI)
20. Wan SW, Lin CF, Wang S, Chen YH, Yeh TM, Liu HS, Anderson R, Lin YS. Current progress in dengue vaccines. J Biomed Sci 2013; 20:37. (invited review article) (highly accessed articles) (SCI)
21. Tsai TT, Chuang YJ, Lin YS, Wan SW, Chen CL, Lin CF. An emerging role for the anti-inflammatory cytokine interleukin-10 in dengue virus infection. J Biomed Sci 2013; 20:40. (invited review article) (SCI)
22. Wan SW, Lin CF, Yeh TM, Liu CC, Liu HS, Wang S, Ling P, Anderson R, Lei HY, Lin YS. Autoimmunity in dengue pathogenesis. J Formos Med Assoc. 2013; 112: 3-11. (invited review article) (SCI)
23. Chen CL, Lin CF, Wan SW, Wei LS, Chen MC, Yeh TM, Liu HS, Anderson R, Lin YS. Anti-dengue virus nonstructural protein 1 antibodies cause NO-mediated endothelial cell apoptosis via ceramide-regulated GSK-3β and NF-κB activation. J Immunol 2013; 191:1744-1752. (SCI)
24. Wan SW, Lin CF, Lu YT, Lei HY, R. Anderson, YS Lin. Endothelial cell surface expression of protein disulfide isomerase activates β1 and β3 integrins and facilitates dengue virus infection. J Cell Biochem 2012; 113: 1681–1691. (SCI)
25. Lin YS, Yeh TM, Lin CF, Wan SW, Chuang YC, Hsu TK, Liu HS, Liu CC, Anderson R, Lei HY. Molecular mimicry between virus and host and its implications for dengue disease pathogenesis. Exp Biol Med 2011; 236: 515-523. (invited review article) (SCI)
26. Cheng HJ, Lei HY, Lin CF, Luo YH, Wan SW, Liu HS, Yeh TM, Lin YS. Anti-dengue virus nonstructural protein 1 antibodies recognize protein disulfide isomerase on platelets and inhibit platelet aggregation. Mol Immunol 2009; 47: 398-406. (SCI)
27. Wan SW, Lin CF, Chen MC, Lei HY, Liu HS, Yeh TM, Liu CC, Lin YS. C-terminal region of dengue virus nonstructural protein 1 is involved in endothelial cell cross-reactivity via molecular mimicry. Am J Infect Dis 2008; 4:85-91. (SJR: 0.2)
28. Lin CF, Wan SW, Chen MC, SC Lin, Cheng CC, Chiu SC, Hsiao YL, Lei HY, Liu HS, Yeh TM, YS Lin. Liver injury caused by antibodies against dengue virus nonstructural protein 1 in a murine model. Lab Invest 2008; 88:1079-1089. (SCI)
29. Lin CF, Wan SW, Chen MC, Lei HY, Lin YS. Increased dengue virus-infected endothelial cell apoptosis caused by antibodies against nonstructural protein 1. Dengue Bull 2007; 31:111-117. (WHO)
30. Lin CF, Wan SW, Cheng HJ, Lei HY, Lin YS. Autoimmune pathogenesis in dengue virus infection. Viral Immunol 2006; 19:127-132. (invited review article) (SCI)
31. Lin CF, Chiu SC, Hsiao YL, Wan SW, Lei HY, Shiau AL, Liu HS, Yeh TM, Chen SH, Liu CC, Lin YS. Expression of cytokine, chemokine, and adhesion molecule during endothelial cell activation induced by antibodies against dengue virus nonstructural protein 1. J Immunol 2005; 174:395-403. (SCI)