Genome-wide survey of protein kinases required for cell cycle progression

Nature. 2004 Dec 23;432 (7020):980-7

Speaker：鄭智仁                  Time：10/26/2005 13:10-14:00

Commentator：劉校生 老師         Place：Room 601

Abstract：

Series of protein phosphorylation are required for progression of cell cycle through division for eukaryotic cells. Genetic studies on model organisms, particularly fungi andDrosophila, have successfully identified cell cycle kinases and assessed their functions. The authors analyzed the complement of Drosophila protein kinases (kinome) for cell cycle functions after gene silencing by RNA interference. Both low redundancy of protein kinases in the Drosophila genome and complete representation of their sub-families in human make it an attractive approach in exploration of cell cycle functions. Cell cycle dysfunction was observed upon downregulation of 80 out of 228 protein kinases, including most kinases known to regulate division cycle. New enzymes were found to have cell cycle functions, and some of their family members are known to phosphorylate microtubules, actin or their associated proteins. Depletion of several signaling kinases leads to specific mitotic aberrations, suggestive of a novel role for these enzymes. The survey reveals the inter-digitation of systems that monitor cellular physiology, cell size, cellular stress and signaling processes related to cell cycle regulation.

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