Mucin–Pseudomonas aeruginosa interactions promote biofilm formation and antibiotic resistance

Mol Microbiol 59: 142-151 (2006)

Speaker：楊朝傑                           Time：2/15/2006, 15:10~16:00

Commentator：何漣漪老師                   Place：Room 601

Abstract：

Pseudomonas aeruginosa is a gram-negative opportunistic pathogen associated with pneumonia in immunocompromised patients and chronic lung disease, such as cystic fibrosis (CF).  CF is the most common, lethal inherited disorder in Caucasian populations.  In CF airways, P. areuginosa forms surface-associated communities called biofilms.  The role of biofilms in CF pathogenesis is important for resistant to antimicrobial agents and inhibition of clearance by the host immune system.  Previous studies have shown that environmental parameters have a significant influence on biofilm development.^1,2  In order to understand CF airway infections are caused by P. areuginosa biofilms, the authors developed a technique to coat surfaces with host-derived polymers in CF airways, such as DNA, actin and glycoprotein mucin.  They found that P. aeruginosa biofilm development proceeds differently on surfaces coated with the mucin compared with biofilm development on glass and surfaces coated with actin or DNA.  Biofilms formed on mucin display distinct, large cellular aggregates that are significantly more resistant to the antibiotic tobramycin than a comparable biofilm grown on glass.  In addition, an analysis of biofilm formation using select mutant backgrounds and time-lapse microscopy indicated that surface-associated motility was blocked on the mucin surface and a specific adhesin-mucin interaction immobilized the bacterium on the surface.  These findings reveal that mucin of CF airways have the potential to influence theP. aeruginosa biofilm development and function.³

References：

1.       P.E. Kolenbrander. Oral microbial communities: biofilms, interactions, and genetic systems. Annu Rev Microbiol 54: 413–437 (2000).

2.       D.P. Bakker, B.R. Postmus, H.J. Busscher, and H.C. van der Mei. Bacterial strains isolated from different niches can exhibit different patterns of adhesion to substrata. Appl Environ Microbiol 70: 3758–3760 (2004).

3.       R.M. Landry, D. An, J.T. Hupp, P.K. Singh, and M.R. Parsek. Mucin–Pseudomonas aeruginosa interactions promote biofilm formation and antibiotic resistance. Mol Microbiol 59: 142-151 (2006).