PPARγ-Mediated Increase in Glucose Availability Sustains Chronic Brucella abortus Infection in Alternatively Activated Macrophages
PPARγ-Mediated Increase in Glucose Availability Sustains Chronic Brucella abortus Infection in Alternatively Activated Macrophages
Mariana N. Xavier, Maria G. Winter, Alanna M. Spees, Andreas B. den Hartigh, Kim Nguyen, Christelle M. Roux, Teane M.A. Silva, Vidya L. Atluri, Tobias Kerrinnes, A. Marijke Keestra, Denise M. Monack, Paul A. Luciw, Richard A. Eigenheer, Andreas J. Bäumler, Renato L. Santos, and Renée M. Tsolis. Cell Host Microbe. (2013) 14, 159-170.
Speaker: Chao-Hui Weng (翁肇徽) Time: 13:00~14:00, Dec. 4, 2013
Commentator: Dr. I-Hsiu Huang (黃一修 老師) Place: Room 601
Abstract:
Brucellosis is a chronic infection causing significant morbidity that requires protracted treatment with multiple antibiotics. The authors’ previous studies indicate that bacterial metabolism as key to chronic Brucella abortus infection (1). They sought to investigate the relative importance of alternatively activated macrophages (AAMs) and classically activated macrophages (CAMs) as niches for persistent infection, and to determine whether the different metabolic programming of these two macrophage populations contributes to chronic infection by B. abortus. In this study, the authors found that AAMs are more abundant during chronic infection, and AAMs provide a niche for persistence of B. abortus. Peroxisome proliferator-activated receptor γ (PPARγ) induce a metabolic shift to increase intracellular glucose availability that promoting enhanced bacterial survival in AAMs. Glucose uptake is important for increased replication of B. abortus in AAMs during chronic infection. The glucose transporter gluP mutant reduced the ability of B. abortus to replicate and survive in AAMs. In conclusion, PPARγ-induced increases in intracellular glucose availability promoting chronic persistence of B. abortus within AAMs, and inhibiting PPARγ may aid in eradicating chronic infection.
References:
1. Hong, P.C., Tsolis, R.M., and Ficht, T.A. (2000). Identification of genes required for chronic persistence of Brucella abortus in mice. Infect. Immun. 68, 4102–4107.
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