A Single Strain of Clostridium butyricum Induces Intestinal IL-10-Producing Macrophages to Suppress Acute Experimental Colitis in Mice

Atsushi Hayashi, Toshiro Sato, Nobuhiko Kamada, Yohei Mikami, Katsuyoshi Matsuoka, Tadakazu Hisamatsu, Toshifumi Hibi, Axel Roers, Hideo Yagita, Toshiaki Ohteki, AkihikoYoshimura,and Takanori Kanai. Cell Host & Microbe (2013) 13, 711–722

Speaker: Yu-ping Hong (洪羽屏)                         Time: 14:00~15:00, Oct. 16, 2013

Commentator: Dr. Lien-I Hor (何漣漪 教授)             Place: Room 601

Abstract:

The human gastrointestinal tract harbors approximately 10¹⁴ commensal bacteria of over 1,000 species. Dysbiosis, the imbalance of the intestinal microbiota, provokes pro-inflammatory responses associated diseases such as colitis. Previous study reported that a complex mixture of 46 strains from Clostridium clusters IV and XIVa promoted the accumulation of interleukin-10(IL-10)-producing induced T regulatory (iTreg) cells, which suppressed colitis in a dextran sodium sulfate (DSS) colitis model (1). Unlike Clostridium clusters IV and XIVa, Clostridium butyricum(CB) belong to Clostridium cluster I, has been used as a probiotic in clinical practice for many years (2). The MIYAIRI 588 strain of C. butyricum does not carry any genes encoding any toxins and virulence factors associated with clostridium or other enteropathogens (3). In the present study, the author demonstrated that administration of the probiotic bacterial strain CB prevented experimental colitis by an IL-10-dependent mechanism. CB directly triggered IL-10 production by intestinal macrophages in inflamed mucosa via the TLR2/MyD88 pathway. The colitis preventing effect of CB in macrophage-specific IL-10-deficient mice was denied, suggesting that induction of IL-10 by intestinal macrophages is important for the protection action of CB.Therefore, CB promotes IL-10 production by intestinal macrophages in inflamed mucosa, playing an important role in preventing experimental colitis in mice.

References:

1. Atarashi, K., Tanoue, T., Shima, T., Imaoka, A., Kuwahara, T., Momose, Y., Cheng, G., Yamasaki, S., Saito, T., Ohba, Y., et al. (2011). Induction of colonic regulatory T cells by indigenousClostridium species. Science 331, 337–341.

2. Seki, H., Shiohara, M., Matsumura, T., Miyagawa, N., Tanaka, M., Komiyama, A., and Kurata, S.(2003). Prevention of  antibiotic-associated  diarrhea in children by Clostridium butyricumMIYAIRI. Pediatr. Int. 45, 86–90.

3.  http://www.food.gov.uk/multimedia/pdfs/acnfp10505clos.pdf