Differenced in Type I Interferon Signaling Antagonism by Dengue viruses in Human and Non-Human primate cell Lines

Freddy A. Medina, Giselle Torres-Malavé, Amanda J. Chase, Gilberto A. Santiago,

Juan F. Medina, Luis M. Santiago, Jorge L. Muñoz-Jordán

Speaker: Sina Taheri Baghmisheh (白明旭)           Time: 15:00~16:00, Jun. 24, 2015

Commentator: Dr. Guey- Chuen Perng (彭貴春 老師)   Place: Room 601

Abstract:

Dengue is a viral illness acquired through the bite of an infected mosquito. This flu-like illness, which in rare instances can be fatal, threatens more than half of the world's population [1]. Bothin vitro and clinical studies looking at how the virus operates have consistently found that the interferon response is modulated by the virus during infection. This study showed that ability of dengue virus (DENV) strains to inhibit phosphorylated signal transducer and activator of transcription (pSTAT1) after IFN-β stimulation and observed that contrary to earlier published reports; all DENVs are capable of inhibiting IFN-α/β signaling [2]. Strains from the DENV-2 sylvatic genotype, which mainly infect non-human primates (NHP), displayed an increased ability to inhibit pSTAT1 compared to the Asian strain16681. Surprisingly, DENVs were only capable of inhibiting pSTAT1 in human cell lines, but not in NHP cell lines. Inhibition of pSTAT1 is observed in both human and NHP primary dendritic cells. These results showed that it is important implications in the use of NHP cell lines for studies of IFN-α/β inhibition by DENV in vitro and may be a relevant consideration when using NHPs for DENV pre-clinical studies [3].

References:

1.         in Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control: New Edition. 2009: Geneva.

2.         Munoz-Jordan, J.L., et al., Inhibition of interferon signaling by dengue virus. Proc Natl Acad Sci U S A, 2003. 100(24): p. 14333-8.

3.         Sharp, T.M., et al., Virus-specific differences in rates of disease during the 2010 Dengue epidemic in Puerto Rico. PLoS Negl Trop Dis, 2013. 7(4): p. e2159.