Signalling pathways associated with IL-6 production and epithelial–mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis

Speaker: Sina Taheri Baghmisheh (白明旭)                        Time:15:00~16:00, Nov. 16.2016

Commentator: Chih- Peng Chang (張志鵬 老師)                Place: Room 601

Abstract 

Trichomonas vaginalis (Tv), a parasitic protozoon causes a sexually transmitted disease, with an estimated 275 million new cases worldwide each year [1]. Trichomonas vaginalis (Tv) has been found in patient tissue of benign prostatic hyperplasia (BPH), and suggested to cause chronic prostatitis [2]. IL-6 is known as one of the important factors of chronic inflammation in prostate cancer. Patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) had higher levels of IL-6 in seminal plasma [3]. Furthermore, inflammatory conditions induced by pathogen infections have been shown to promote epithelial–mesenchymal transition (EMT)[5]. In this article they investigated the signals involved in IL-6 production by human prostate epithelial cells (PECs) stimulated with Tv and examined whether Tv induces EMT in PECs. The author found that PECs stimulated with Tv increased the expression of IL-6, as well as the expression of TLR2, TLR4, MAPKs (p38, JNK, ERK), NF-κB and JAK2/STAT3, and levels of ROS. Inhibition of TLR2 or TLR4 reduced IL-6 production as well as expression of these other factors, and agents inhibiting ROS, MAPKs, NF-κB and JAK reduced IL-6 production. However, when PECs were stimulated with Tv, transcripts of mesenchymal cell markers increased, and epithelial cell markers decreased. In addition, the induction of EMT was suppressed by inhibitors of JAK or NF-κB. These findings are the first evidence that Tv infection of prostate epithelial cells may induce EMT.

Reference:

1.      World Health Organization. Prevalence and Incidence of Selected Sexually Transmitted Infections, Chlamydia trachomatis, Neisseria gonorrhoeae, syphilis, and Trichomonas vaginalis.

2.      Skerk V, Schonwald S, Krhen I, et al. Aetiology of chronic prostatitis. Int J Antimicrob Agents. 2002;19:471–474.

3.      Nguyen DP, Li J, Tewari AK. Inflammation and prostate cancer: the role of interleukin 6 (IL-6). BJU Int. 2014;113:986–992. 

4.      De Craene B, Berx G. Regulatory networks defining EMT during cancer initiation and progression. Nat Rev Cancer. 2013;13:97–110.