A Single Strain of Clostridium butyricum Induces Intestinal IL-10-Producing Macrophages to Suppress Acute Experimental Colitis in Mice

Atsushi Hayashi, Toshiro Sato, Nobuhiko Kamada, Yohei Mikami, Katsuyoshi Matsuoka, Tadakazu Hisamatsu, Toshifumi Hibi, Axel Roers, Hideo Yagita, Toshiaki Ohteki, 

Akihiko Yoshimura, & Takanori Kanai. Cell Host Microbe (2013) 13, 711-722.

Speaker: Yi-Chun Chen (陳怡君)                                Time: 13:10~14:00, Oct. 12, 2016

Commentator: Dr. Jenn-Wei Chen (陳振暐博士)        Place: Room 601

Abstract:

Intestinal microbiota contribute to many host physiological processes, including nutrient acquisition and development of the gut-specific immune system, as well as protection from infectious pathogens. The host in turn provides niches and nutrients for the commensal bacteria (1). Previously study has revealed that an imbalance in the bacterial composition (dysbiosis) provokes host proinflammatory immune responses and induces various inflammatory and metabolic diseases (2). A complex mixture of 46 strains of Clostridium induced TGF-β in intestinal epithelial cells that promoted the interleukin 10 -producing Treg cell accumulation which suppressed symptoms in a dextran sodium sulfate (DSS) colitis mice. (3). However, T cells might be dispensable for acute DSS colitis, bringing into question the role of Treg cells in the probiotic effects of Clostridium species. In this study, the authors demonstrated that administration of the probiotic bacterial strain Clostridium butyricum (CB) prevented experimental colitis via an IL-10-dependent mechanism, the TLR2/MyD88 pathway. CB induced IL-10 production by intestinal macrophages, but not CD4⁺ T cells, in inflamed mucosa, thereby suppressing experimental colitis by an IL-10-dependent mechanism.

References:

1. Garrett, W.S., Gordon, J.I., & Glimcher, L.H. (2010) Homeostasis and inflammation in the intestine. Cell 140, 859-870.

2. Garrett, W.S., Lord, G.M., Punit, S., et al. (2007) Communicable ulcerative colitis induced by T-bet deficiency in the innate immune system. Cell 131, 33-45

3. Atarashi, K., Tanoue, T., Shima, T., et al. (2011) Induction of colonic regulatory T cells by indigenous Clostridium species. Science 331, 337-341