Human Induced Pluripotent Stem Cell-Derived Microvesicles Transmit RNAs and Proteins to Recipient Mature Heart Cells Modulating Cell Fate and Behavior

Sylwia BW, Katarzyna K, Malgorzata S, Sylwia KK, Elzbieta K, Marta A, Urszula J, Anna MT, Michal S, Miroslaw BM, Jacek K, Dariusz B, Zbigniew M, Buddhadeb D, Ewa K. ZS*

Stem cells 2015 Sep; 33(9): 2748-61.

Speaker: Ko-Lun, Yen (顏克倫)                                           Time: 14:10~15:00, Feb, 24, 2016

Commentator: Dr. Chia-Ching Wu (吳佳慶 副教授)         Place: Room 601

Abstract:

Microvesicles (MVs) are membrane-enclosed cytoplasmic which are shed from the surface membrane of activated cells. An increase or decrease in MV release and changes in their composition have been recognized as important components of the intercellular communication and associating with many pathological conditions. Therefore, monitoring MV release has been suggested as a prognostic marker of various disease, particularly in cancer. Apart from the diagnostic use, an increasing evidence suggests that MVs may involve in tissue regeneration^[1]. Induced pluripotent stem cells (iPSCs), an artificial cell type of pluripotent stem cell which has the ability to be tailor-made into various cell types indefinitly, are being increasingly recognized, cumultive evidence suggests that iPSCs can serve as a good source for transplantation and regeneration medicine. Recent studies have indicated that embryonic stem cells (ESCs), the most well-known pluripotent stem cell, are able to reprogram hematopoietic progenitors and revert them to a more primitve state^[2]. However, the utilizing of iPSC-derived MVs in this regard remains unknown. In this study, the authors investigated the effect of human iPSC-MVs on mature heart cells, and analysed the transcriptome and proteomic profile comprehensively both in iPSC-MVs and target cells. For the first time, the author observed hiPSCs-derived MVs, which carried stem cell specific components, are able to exert beneficial effects on both mature and primitive stem cells, providing a new iPS cell-based strategies in tissue regeneration by applicate their safe acellular bioactive derivatives.

Reference:

1.     Ranghino A, Cantaluppi V, Grange C et al,. Endothelial progenitor cell-derived microvesicles improve neovascularization in a murine model of hindlimb ischemia. Int J Immunopathol Pharmacol 2012; 25:75-85.

2.     Ratajcazak J, Miekus K, Kucia M et al,. Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: Evidence for horizontal transfer of mRNA and protein delivery. Leukemia 2006;20:847-856.