Immunomodulatory potential of particular Trichinella spiralis muscle larvae excretory-secretory components

Cvetkovic J., Sofronic-Milosavljevic L., Ilic N., Gnjatovic M., Nagano I., Gruden-Movsesijan A.

Int J Parasitol. (2016) 46, 833-842.

Speaker: Yi-Chun Chen (陳怡君)                                Time: 14:10~15:00, Mar. 22, 2017

Commentator: Dr. Jyh-Wei Shin (辛致煒 博士)         Place: Room 601

Abstract:

The parasitic nematode Trichinella spiralis causes chronic infection in a wide range of mammalian hosts including humans (1). The anti-inflammatory environment created by this parasite hinders development of inflammatory disorders such as autoimmune diseases (2). However, there is a great need for the exploration of mechanisms and molecules used by parasites with the aim of gathering knowledge which might help in development of new treatments for inflammatory diseases. The impact of T. spiralis on cells and molecules of the host’s immune system is achieved through excretory–secretory products (ES L1) released from larvae encapsulated in muscle. ES L1 induced tolerogenic DCs that are responsible for triggering Th2 and anti-inflammatory responses, accompanied by activation of regulatory T cells (3). In this study, the authors focus on the role of individual ES L1 components that drive such responses, as well as the molecular pathways triggered by interaction of the ES L1 components and the host immune cells. They explore the role of the ES L1-derived triplet containing 45, 49 and 53 kDa glycoproteins, isolated on the basis of the specificity of 7C2C5 mAb, 7C2C5 Antigen (7C2C5Ag) and recombinant protein 53 of T. spiralis (rTsp53) in the induction and polarization of the host immune response. These results revealed that both 7C2C5Ag and rTsp53 caused partial maturation of DCs, accompanied by elevated IL-10 and unchanged IL-12p70 production compared with the cytokine level of unstimulated cells. DCs primed with 7C2C5Ag or rTsp53 skewed polarization of T cells towards the Th2/anti-inflammatory type of response. It could be concluded that the investigated ES antigens components can largely reproduce the immunomodulatory effects and therefore may be considered as molecules important for creation of the anti-inflammatory environment achieved by the parasite.

References:

1.    Ilic N., Gruden-Movsesijan A., Sofronic-Milosavljevic L. (2012) Trichinella spiralis: shaping the immune response. Immunol Res. 52, 111-119

2.    Gruden-Movsesijan A., Ilic N., Mostarica-Stojkovic M., et al. (2010) Mechanisms of modulation of experimental autoimmune encephalomyelitis by chronic Trichinella spiralis infection in Dark Agouti rats. Parasite Immunol. 32, 450-459

3.    Gruden-Movsesijan A., Ilic N., Colic M., et al. (2011) The impact of Trichinella spiralis excretory-secretory products on dendritic cells. Comp Immunol Microbiol Infect Dis. 34, 429-439