小檔案

學 歷

成功大學基礎醫學研究所博士 (2004-2012)

成功大學微生物及免疫學研究所碩士 (2002-2004)

高雄醫學大學醫學檢驗生物學系學士 (1998-2002)

經 歷

成功大學微生物及免疫學研究所助理教授 (2021-present)

義守大學學士後醫學系外國學生專班助理教授 (2017-2021)

成功大學傳染性疾病及訊息研究中心博士後研究員 (2012-2017)

專長學門

免疫學

病毒學

細胞生物學

研究興趣

登革病毒感染是以節肢動物傳播的病毒疾病中主要的原因之一。截至目前為止，尚無針對登革病毒的抗病毒藥物或治療方法獲得許可。病毒蛋白中的非結構性蛋白1 (NS1) 在登革病毒感染中扮演重要角色；細胞內的角色有幫助病毒複製和病毒顆粒的產生，細胞外的角色則是參與在血漿滲漏與免疫逃避。因此，我的研究興趣專注於非結構性蛋白1的致病機制與發展具潛力的臨床應用。

實驗室主要研究方向為：

• 非結構性蛋白1在調控細胞內環境恆定的角色。
• 非結構性蛋白1的分泌路徑。
• 以非結構性蛋白1為基礎，發展有效且可廣泛應用的次單位疫苗與治療性抗體。

PUBLICATION

Refereed papers

1. Tien SM, Chang PC, Lai YC, Chuang YC, Tseng CK, Kao YS, Huang HJ, Hsiao YP, Liu YL, Lin HH, Chu CC, Cheng MH, Ho TS, Chang CP, Ko SF, Shen CP, Anderson R, Lin YS*, Wan SW*, Yeh TM*. Therapeutic efficacy of humanized monoclonal antibodies targeting dengue virus nonstructural protein 1 in the mouse model. PLoS Pathog 2022; 18: e1010469. (*equal contribution as the corresponding author)
2. Huang HJ, Yang M, Chen HW, Wang S, Chang CP, Ho TS, Kao YS, Tien SM, Lin HH, Chang PC, Lai YC, Hsiao YP, Liu YL, Chao CH, Anderson R, Yeh TM, Lin YS*, Wan SW*. A novel chimeric dengue vaccine candidate composed of consensus envelope protein domain III fused to C-terminal-modified NS1 protein. Vaccine 2022; 40: 2299-2310. (*equal contribution as the corresponding author)
3. Wan SW, Lee YR, Ho TS, Chang CP. Regulation of innate immune signaling pathways by autophagy in dengue virus infection. IUBMB Life 2022; 74:170-179. (SCI)
4. Chang CJ, Lin CF, Lee CH, Chuang HC, Shih FC, Wan SW, Tai C, Chen CL. Overcoming interferon (IFN)-γ resistance ameliorates transforming growth factor (TGF)-β-mediated lung fibroblast-to-myofibroblast transition and bleomycin-induced pulmonary fibrosis. Biochem Pharmacol 2021; 183:114356. (SCI)
5. Lu ZY, Cheng MH, Yu CY, Lin YS, Yeh TM, Chen CL, Chen CC, Wan SW*, Chang CP*. Dengue nonstructural protein 1 maintains autophagy through retarding caspase-mediated cleavage of beclin-1. Int J Mol Sci 2020; 21:9702. (*equal contribution as the corresponding author) (SCI)
6. Kao YS, Yu CY, Huang HJ, Tien SM, Wang WY, Yang M, Anderson R, Yeh TM, Lin YS*, Wan SW*. Combination of modified NS1 and NS3 as a novel vaccine strategy against dengue virus infection. J Immunol 2019; 203:1909-1917. (*equal contribution as the corresponding author) (SCI)
7. Tseng PC, Kuo CF, Cheng MH, Wan SW, Lin CF, Chang CP, Lin YS, Wu JJ, Huang CC and Chen CL. HECT E3 ubiquitin ligase-regulated Txnip degradation facilitates TLR2-mediated inflammation during group A streptococcal infection. Front Immunol 2019; 10:2147. (SCI)
8. Wan SW, Wu-Hsieh BA, Lin YS, Chen WY, Huang Y, Anderson R. The monocyte-macrophage-mast cell axis in dengue pathogenesis. J Biomed Sci 2018; 25:77. (invited review article) (SCI)
9. Chiu YH, Chen MC, Wan SW. Sodium hyaluronate/chitosan composite microneedles as a single-dose intradermal immunization system. Biomacromolecules 2018; 19:2278-2285. (SCI)
10. Wan SW*, Chen PW*, Chen CY, Lai YC, Chu YT, Hung CY, Lee H, Wu H, Chuang YC, Lin J, Chang CP, Wang S, Liu CC, Ho TS, Lin CF, Lee CK, Anderson R, Yeh TM, Lin YS. Therapeutic effects of monoclonal antibody against dengue virus NS1 in a STAT1 knockout mouse model of dengue infection. J Immunol 2017; 199:2834-2844. (*equal contribution as the 1st author) (SCI)
11. Chu YT*, Wan SW*, Chang YC, Lee CK, Wu-Hsieh BA, Anderson R, Lin YS. Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation. Lab Invest 2017; 97: 602-617. (*equal contribution as the 1st author) (SCI)
12. Zainal N, Chang CP, Cheng YL, Anderson R, Wan SW, Ho TS, AbuBakar S, Lin YS. Resveratrol treatment reveals a novel role for HMGB1 in regulation of the type 1 interferon response in dengue virus infection. Sci Rep 2017; 7: 42998. (SCI)
13. Wan SW*, Yang YW*, Lin CF, Chang CP, Yeh TM, Anderson R, Lin YS. Anti-dengue virus nonstructural protein 1 antibodies contribute to platelet phagocytosis by macrophages. Thromb Haemost 2016; 115: 646-656. (*equal contribution as the 1st author) (SCI)
14. Chu YT, Wan SW, Anderson R, Lin YS. Mast cell-macrophage dynamics in modulation of dengue virus infection in skin. Immunology 2015; 146: 163-172. (SCI)
15. Cheng YL, Lin YS, Chen CL, Wan SW, Ou YD, Yu CY, Tsai TT, Tseng PC, Lin CF. Dengue virus infection causes the activation of distinct NF-κB pathways for inducible nitric oxide synthase and TNF-α expression in RAW264.7 cells. Mediat Inflamm 2015; 2015:274025. (SCI)
16. Cheng HJ, Luo YH, Wan SW, Lin CF, Wang ST, Hung NT, Liu CC, Ho TS, Liu HS, Yeh TM, Lin YS. Correlation between serum levels of anti-endothelial cell autoantigen and anti-dengue virus nonstructural protein 1 antibodies in dengue patients. Am J Trop Med Hyg 2015; 92: 989-995. (SCI)
17. Tsai TT, Chuang YJ, Lin YS, Chang CP, Wan SW, Lin SH, Chen CL, Lin CF. Antibody-dependent enhancement infection facilitates dengue virus-regulated signaling of IL-10 production in monocytes. PLoS Negl Trop Dis 2014; 8: e3320. (SCI)
18. Fang YT, Wan SW, Lu YT, Yao JH, Lin CF, Hsu LJ, Brown MG, Marshall JS, Anderson R, Lin YS. Autophagy facilitates antibody-enhanced dengue virus infection in human pre-basophil/mast cells. PLoS One 2014; 9: e110655. (SCI)
19. Wan SW, Lu YT, Huang CH, Lin CF, Anderson R, Liu HS, Yeh TM, Yen YT, Wu-Hsieh BA, Lin YS. Protection against dengue virus infection in mice by administration of antibodies against modified nonstructural protein 1. PLoS One 2014; 9: e92495. (SCI)
20. Wan SW, Lin CF, Wang S, Chen YH, Yeh TM, Liu HS, Anderson R, Lin YS. Current progress in dengue vaccines. J Biomed Sci 2013; 20:37. (invited review article) (highly accessed articles) (SCI)
21. Tsai TT, Chuang YJ, Lin YS, Wan SW, Chen CL, Lin CF. An emerging role for the anti-inflammatory cytokine interleukin-10 in dengue virus infection. J Biomed Sci 2013; 20:40. (invited review article) (SCI)
22. Wan SW, Lin CF, Yeh TM, Liu CC, Liu HS, Wang S, Ling P, Anderson R, Lei HY, Lin YS. Autoimmunity in dengue pathogenesis. J Formos Med Assoc. 2013; 112: 3-11. (invited review article) (SCI)
23. Chen CL, Lin CF, Wan SW, Wei LS, Chen MC, Yeh TM, Liu HS, Anderson R, Lin YS. Anti-dengue virus nonstructural protein 1 antibodies cause NO-mediated endothelial cell apoptosis via ceramide-regulated GSK-3β and NF-κB activation. J Immunol 2013; 191:1744-1752. (SCI)
24. Wan SW, Lin CF, Lu YT, Lei HY, R. Anderson, YS Lin. Endothelial cell surface expression of protein disulfide isomerase activates β1 and β3 integrins and facilitates dengue virus infection. J Cell Biochem 2012; 113: 1681–1691. (SCI)
25. Lin YS, Yeh TM, Lin CF, Wan SW, Chuang YC, Hsu TK, Liu HS, Liu CC, Anderson R, Lei HY. Molecular mimicry between virus and host and its implications for dengue disease pathogenesis. Exp Biol Med 2011; 236: 515-523. (invited review article) (SCI)
26. Cheng HJ, Lei HY, Lin CF, Luo YH, Wan SW, Liu HS, Yeh TM, Lin YS. Anti-dengue virus nonstructural protein 1 antibodies recognize protein disulfide isomerase on platelets and inhibit platelet aggregation. Mol Immunol 2009; 47: 398-406. (SCI)
27. Wan SW, Lin CF, Chen MC, Lei HY, Liu HS, Yeh TM, Liu CC, Lin YS. C-terminal region of dengue virus nonstructural protein 1 is involved in endothelial cell cross-reactivity via molecular mimicry. Am J Infect Dis 2008; 4:85-91. (SJR: 0.2)
28. Lin CF, Wan SW, Chen MC, SC Lin, Cheng CC, Chiu SC, Hsiao YL, Lei HY, Liu HS, Yeh TM, YS Lin. Liver injury caused by antibodies against dengue virus nonstructural protein 1 in a murine model. Lab Invest 2008; 88:1079-1089. (SCI)
29. Lin CF, Wan SW, Chen MC, Lei HY, Lin YS. Increased dengue virus-infected endothelial cell apoptosis caused by antibodies against nonstructural protein 1. Dengue Bull 2007; 31:111-117. (WHO)
30. Lin CF, Wan SW, Cheng HJ, Lei HY, Lin YS. Autoimmune pathogenesis in dengue virus infection. Viral Immunol 2006; 19:127-132. (invited review article) (SCI)
31. Lin CF, Chiu SC, Hsiao YL, Wan SW, Lei HY, Shiau AL, Liu HS, Yeh TM, Chen SH, Liu CC, Lin YS. Expression of cytokine, chemokine, and adhesion molecule during endothelial cell activation induced by antibodies against dengue virus nonstructural protein 1. J Immunol 2005; 174:395-403. (SCI)