|
系統性紅斑性狼瘡是多種器官遭受破壞的自體免疫行疾病,除了免疫系統
調控失常外,並且伴隨著多發性的自體免疫抗體的產生。病人具有過活化
的自體免疫T細胞及B細胞對抗自體抗原,並且T細胞對病情的完全發展具有
關鍵的重要性。但是以往的研究結果証實病人週邊T細胞的許多功能比正
常人的T細胞功能還要低。本論文想要探討的是系統性紅斑性狼瘡病人血
清中異常的因子是否干擾T細胞的功能。實驗系統是利用兩株人類T細胞株
MOLT-4及Jurkat培養在以protein-A affinity column移除大部分抗淋巴
球抗體的病人血清裡後,檢測其生長及死亡的表現。結果顯示MOLT-4及
Jurkat在病人血清的處理下,其生長速率減低,以trypan blue測得細胞死
亡率及以cell death ELISA測得的細胞計劃性死亡皆增加。以Western
blotting測得Bcl-2的表現,在病人血清的培養下與正常控制組沒有不同。
而病人血清的處理也不會影響MOLT-4細胞週期的進行。綜合這些實驗結果
所示,系統性紅斑性狼瘡病人血清含有某些因子不會影響細胞週期的進行,
但卻會處促成較多的細胞死亡。這可能是病人血清造成MOLT-4及Jurkat生
長速率減低的原因。
|
|
Systemic lupus erthematosus (SLE) is a multisystem disease
characterized by disturbances in the immune system associated
with the production of autoantibodies to self-antigens.
Autoreactive B-cells and T cells are hyperactivated in patients
with SLE.T-cell help is thought to be critical for the
development of full-blown disease. But an interesting paradox
occurs, in that hypoactivityof many functions measured in
peripheral T lymphocytes have been identified. In this study
whether SLE serum factors removed of anti-lymphocyte IgG are
reponsible for the aberrant functions of T cells of patients
with SLE was tested with an in vitro culture system. Two human
leukemia T cell line, MOLT-4 and Jurkat, were treated with SLE
sera removed of anti-lymphocyte antibodies via a Protein-A
column. Removed showed that MOLT-4 and Jurkat cells had a
slower growth rate in media containing SLE sera as compared to
normal serum control. A higher death ratio and increased
apoptosis level as measured by trypan blue exclusion, cell
death detection ELISA were observed in MOLT-4 and Jurkat
stimulated by SLE sera. The Bcl-2 expression detected by
Western blotting was not affected by SLE sera. Moreover, MOLT-4
and Jurkat cultured in SLE sera showed a similar pattern of
cell cycle progression as normal control. In conclusion, these
data indicated that SLE sera contain some factors which did not
influence the cell cycle progression but trigger an accelerated
apoptosis and that resulted in a decrease proliferation of
MOLT-4 and Jurkat.
|
